miR-588 suppresses SCC cell migration and invasion. Representative results of Transwell assays of the (A) migration and (B) invasion abilities of H226 and H2170 cells transfected with miR-588 mimics (magnification, ×200). Results of the migration and invasion assays are shown as the average number of cells from five randomly selected fields. Data are presented as the mean ± standard error of the mean of three independent experiments. (C) Migratory and invasive behaviors were analyzed following the inhibition of miR-588 in H1703 cells (magnification, ×200). Quantification of cell migration and invasion was performed from five randomly selected fields. Each experiment was performed in triplicate and data are expressed as the mean ± standard error of the mean (*P<0.05, **P<0.01 and ***P<0.001). miR, microRNA; SCC, squamous cell carcinoma; NC, negative control.