Effects of AAFE on serum levels of IL-4, IL-6, IFN-γ and IL-1β, and mRNA expression levels of IL-4, IL-6, IL-13, IFN-γ, IL-1β, TNF-α and GM-CSF in skin-derived lymph nodes from a mouse model of AD-like lesions. (A) Serum IL-4, IL-6, IFN-γ and IL-1β levels. Data presented as the mean ± standard deviation from five animals. Data were analyzed using one-way analysis of variance followed by Dunnett's post-hoc multiple comparison test. ***P<0.001 vs. the control group. (B) Representative IL-4, IL-6, IL-13, IFN-γ, IL-1β, TNF-α and GM-CSF mRNA expression levels. AAFE, Artemisia argyi Folium extract; IL, interleukin; IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α; GM-CSF, granulocyte-macrophage colony-stimulating factor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.