Table I.
Summary of the gene-specific polymerase chain reaction primer sequences, product size and annealing temperature.
| Description | Genebank | Sense primer, 5′-3′ | Antisense primer, 5′-3′ | Product size, bp | Tm, °C |
|---|---|---|---|---|---|
| β-actin | NM_031144 | CGTTGACATCCGTAAAGACCTC | TAGGAGCCAGGGCAGTAATCT | 110 | 58 |
| Cyp3a1 | NM_013105.2 | ACTGCATTGGCATGAGGTTTG | ATCCCGTGGCACAACCTTT | 170 | 58 |
| Cyp3a2 | NM_153312.2 | ATTCTAAGCATAAGCACCGAGTG | TGTGCTGCTGGTGGTTTCAT | 158 | 58 |
| Ugt1a1 | NM_012683.2 | ACTATTCTTGTCAAATGGCTACCC | GTTTTCCAAATCATCGGCAGT | 231 | 58 |
| Slco2b1 | NM_080786.1 | TCGCTGTTGTGTCTGCTACTCAG | AACAGGGTTAAAGTCATCTGATTGG | 162 | 58 |
| Slco1b2 | NM_031650.3 | TTCGTGGTGATAAGAAGCCG | CAATTCAGGTTGGACGCTCTT | 162 | 58 |
| Mdr1 | NM_012623.2 | TCCTATGCTGCTTGTTTCCG | ATCCTGATGATGTGGGATGCT | 179 | 58 |
| Bcrp1 | NM_181381.2 | ATTGGTGCCCTTTACTTTGGTC | ACACTTGGCAAGAACCTCATAGG | 236 | 58 |
| Mrp2 | NM_012833.2 | TGTGGCAGTTGAGCGAATAAGT | AAGAGGCAGTTTGTGAGGGATG | 246 | 58 |
Tm, annealing temperature; Cyp, cytochrome P; Ugt1a1, UDP glucuronosyltransferase family 1 member A complex locus; Slco, solute carrier organic anion transporter family member; Mdr1, multi-drug resistance 1; Bcrp, breast cancer resistance protein; Mrp2, multidrug resistance-associated protein 2.