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. 2016 Sep 6;14(4):3662–3668. doi: 10.3892/mmr.2016.5729

Figure 2.

Figure 2

ICC and Alizarin Red S staining during osteogenic differentiation. Commercial osteogenic differentiation medium was used to induce the osteoblastic differentiation of MC3T3-E1 cells. (A) ICC was performed on day 14 to detect Alp and OC. Negative control cells were not stained for Alp and OC. Magnification, ×400. (B) Alizarin Red S staining was performed on days 0, 3, 5, 7 and 14 to determine the extent of matrix mineralization. Magnification, ×200. Data are presented as the mean ± standard error (n=10). *P<0.05 and **P<0.01 vs. day 0. ICC, immunocytochemistry; NC, negative control; Alp, alkaline phosphatase; OC, osteocalcin.