Skip to main content
. 2016 Aug 9;14(4):3152–3158. doi: 10.3892/mmr.2016.5615

Figure 5.

Figure 5

Effects of HVC1 on mRNA expression of PPAR-γ, HMG-CoA R and LDLR, and the activation of AMPK in the liver tissue. Total RNA was prepared for the real-time-PCR analysis of (A) PPAR-γ, (B) HMG-CoA R and (C) LDLR gene expression from liver tissues. Reverse transcription-quantitative polymerase chain reaction analysis was conducted using a Step One Plus Real-time PCR system. (D) The liver tissue was homogenized and total protein was prepared. Western blot analysis was performed using specific antibodies. β-actin was used as an internal control. ND, Normal diet group; HCD, High-cholesterol diet group; Sim, Simvastatin (10 mg/kg) treated with HCD group; HVC1, HVC1 treated with HCD group. The values are represented as the mean ± standard error of the mean (n=6). #P<0.05, compared to ND; *P<0.05, **P<0.01 and ***P<0.001 compared to HCD. PPAR-γ, peroxisome proliferator-activated receptor-γ; HMG-CoA R, 3-hydroxy-3-methylglutaryl-CoA reductase; LDLR, low-density lipoprotein receptor; AMPK, AMP-activated protein kinase; p-, phosphorylated.