Figure 2.

ARC inhibits apoptosis of human osteoblasts. (A) Cell apoptosis was detected by flow cytometry. One representative image from 3 independently performed experiments is presented. (B) Cell apoptosis was significantly reduced in ARC-overexpressed human osteoblasts compared with empty vector-transfected controls on day 3 under 0, 1 and 5 µM concentrations of Zoledronate treatment. ^*P<0.05, ^**P<0.01 vs. empty vector-transfected controls. (C) Western blot analysis identified reduced expression of cleaved-caspase-3 in ARC-overexpressed human osteoblasts compared with empty vector-transfected controls on day 7 under 0, 1 and 5 µM concentrations of Zoledronate treatment. ARC, apoptosis repressor with caspase recruitment domain; PI, propidium iodide; OB, osteoblast; con, control; nol, Zoledronate.