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. 2016 Aug 11;14(4):3104–3112. doi: 10.3892/mmr.2016.5621

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Copyright: © Ghazaryan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Amniotic membrane with cultured stem cells under confocal microscope (magnification, ×40). (A) Normal amniotic membrane with epithelial layer, (B) epithelial cell nuclei stained with DAPI demonstrated regular arrangement; (C) denuded amniotic membrane with (D) no visible DAPI stained nuclei; (E) amniotic membrane with cultured stem cells; (F) nuclei of stem cells stained with DAPI demonstrated a irregular arrangement; (G) stem cells expressing GFP protein; (H) GFP-positive cells with DAPI stained nuclei. DAPI, 4′,6-diamidino-2-phenylindole; GFP, green fluorescent protein.