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. 2016 Aug 11;14(4):3104–3112. doi: 10.3892/mmr.2016.5621

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Copyright: © Ghazaryan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Corneal flat mounts under light microscope (magnification, ×10). (A) Subconjunctival injection group (group 1), vessels are short and only in one quadrant; (B) amniotic membrane transplantation group (group 2), vessels are in more than half of the cornea and reach to the center; (C) control group (group 3), vessels are over the entire cornea; (D) VEGF protein level detected by ELISA; (E) length of the vessels measured by Image-Pro Plus 6.0; (F) VEGF, (G) MMP-9, (H) TLR2 and (I) TLR4 gene expression relative to actin. ^*P≤0.05, 95% confidence interval. VEGF, vascular endothelial growth factor.