Table I.

The mRNA sequence of different shRNA-HDAC short fragment.

Index	Primer sequence
shRNA-HDAC1-1	F: 5′-CACCGCTCCATCCGTCCAGATAACACGAATGTTATCTGGACGGATGGAGC-3′
	R: 5′-AAAAGCTCCATCCGTCCAGATAACATTCGTGTTATCTGGACGGATGGAGC-3′
shRNA-HDAC1-2	F: 5′-CACCGCAAGCAGATGCAGAGATTCACGAATGAATCTCTGCATCTGCTTGC-3′
	R: 5′-AAAAGCAAGCAGATGCAGAGATTCATTCGTGAATCTCTGCATCTGCTTGC-3′
shRNA-HDAC1-NC	F: 5′-CACCGCAATTTTTTTTTTTGATTCACGAATGAATCAAAAAAAAAAAAATGC-3′
	R: 5′-AAAAGCAATTTTTTTTTTTGATTCATTCGTGAATCAAAAAAAAAAAAATGC-3′
shRNA-HDAC2-1	F: 5′-CACCGCAGATGCAGAGATTTAATGTCGAAACATTAAATCTCTGCATCTGC-3′
	R: 5′-AAAAGCAGATGCAGAGATTTAATGTTTCGACATTAAATCTCTGCATCTGC-3′
shRNA-HDAC2-2	F: 5′-CACCGGCTGGAGGATTACATCATGCCGAAGCATGATGTAATCCTCCAGCC-3′
	R: 5′-AAAAGGCTGGAGGATTACATCATGCTTCGGCATGATGTAATCCTCCAGCC-3′
shRNA-HDAC2-NC	F: 5′-CACCGCAATTTTTTTTTTTGATTCACGAATGAATCAAAAAAAAAAAAATGC-3′
	R: 5′-AAAAGCAATTTTTTTTTTTGATTCATTCGTGAATCAAAAAAAAAAAAATGC-3′
shRNA-HDAC4-1	F: 5′-CACCGGAGCTCGTGGTACTCAAACACGAATGTTTGAGTACCACGAGCTCC-3′
	R: 5′-AAAAGGAGCTCGTGGTACTCAAACATTCGTGTTTGAGTACCACGAGCTCC-3′
shRNA-HDAC4-2	F: 5′-CACCGGAACACATCAAGCACCAACACGAATGTTGGTGCTTGATGTGTTCC-3′
	R: 5′-AAAAGGAACACATCAAGCACCAACATTCGTGTTGGTGCTTGATGTGTTCC-3′
shRNA-HDAC4-NC	F: 5′-CACCGCAATTTTTTTTTTTGATTCACGAATGAATCAAAAAAAAAAAAATGC-3′
	R: 5′-AAAAGCAATTTTTTTTTTTGATTCATTCGTGAATCAAAAAAAAAAAAATGC-3′
shRNA-HDAC6-1	F: 5′-CACCGCAATGGAAGAAGACCTAATCCGAAGATTAGGTCTTCTTCCATTGC-3′
	R: 5′-AAAAGCAATGGAAGAAGACCTAATCTTCGGATTAGGTCTTCTTCCATTGC-3′
shRNA-HDAC6-2	F: 5′-CACCGGAAGAGCTGATGTTGGTTCACGAATGAACCAACATCAGCTCTTCC-3′
	R: 5′-AAAAGGAAGAGCTGATGTTGGTTCATTCGTGAACCAACATCAGCTCTTCC-3′
siRNA-HDAC6-NC	F: 5′-CACCGCAATTTTTTTTTTTGATTCACGAATGAATCAAAAAAAAAAAAATGC-3′
	R: 5′-AAAAGCAATTTTTTTTTTTGATTCATTCGTGAATCAAAAAAAAAAAAATGC-3′
β-actin	F: 5′-GTGGACATCCGCAAAGAC-3′
	R: 5′-AAAGGGTGTAACGCAACTA-3′

shRNA, short hairpin RNA; HDAC, histone deacetylase; NC, negative control; F, forward; R, reverse.