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. 1992 Jun;76(6):338–341. doi: 10.1136/bjo.76.6.338

Synthesis of interleukin-1 and prostaglandin E2 by lens epithelial cells of human cataracts.

O Nishi 1, K Nishi 1, M Imanishi 1
PMCID: PMC504281  PMID: 1622944

Abstract

To test our hypothesis that pseudophakic inflammation, including the fibrin reaction, may be caused by cytokines, prostaglandins (PG), or both, synthesised by residual lens epithelial cells (LECs), we measured interleukin-1 alpha (IL-1 alpha) and PGE2 in the incubation medium of cultures of human LECs obtained by capsulotomy during cataract surgery. After 1 week radioimmunoassay showed that there were 1.46 (0.62) ng of PGE2/10(6) cells (mean (SD) six cultures), and after 4 weeks, there were 5.50 (2.20) ng of PGE2/10(6) cells (seven cultures). During culture the cells proliferated and underwent fibroblast-like cell changes on exposure to the plastic of the wells. In the medium of control plates to which sodium diclofenac had been added PGE2 was not detected. Some IL-1 alpha was found in four of 10 samples, each of which contained media from 12 cultures; 207 pg/10(6) cells in one of the two pools of 2-week cultures, 120 pg/10(6) cells in one pool and 139 pg/10(6) cells in another of the three pools of 3-week cultures, and 111 pg/10(6) cells in the one pool of 4-week cultures. PGE2 and IL-1 alpha may therefore be produced in vivo by residual LECs after cataract surgery, and may be involved in postoperative inflammation, including the fibrin reaction.

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Selected References

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  1. AMBACHE N. Irin, a smooth-muscle contracting substance present in rabbit iris. J Physiol. 1955 Sep 28;129(3):65–6P. doi: 10.1113/jphysiol.1955.sp005387. [DOI] [PubMed] [Google Scholar]
  2. ANGGARD E., SAMUELSSON B. SMOOTH MUSCLE STIMULATING LIPIDS IN SHEEP IRIS. THE IDENTIFICATION OF PROSTAGLANDIN F 2A. PROSTAGLANDINS AND RELATED FACTORS 21. Biochem Pharmacol. 1964 Feb;13:281–283. doi: 10.1016/0006-2952(64)90146-7. [DOI] [PubMed] [Google Scholar]
  3. Bhattacherjee P., Eakins K. E. Inhibition of the prostaglandin synthetase systems in ocular tissues by indomethacin. Br J Pharmacol. 1974 Feb;50(2):227–230. doi: 10.1111/j.1476-5381.1974.tb08565.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chang J., Gilman S. C., Lewis A. J. Interleukin 1 activates phospholipase A2 in rabbit chondrocytes: a possible signal for IL 1 action. J Immunol. 1986 Feb 15;136(4):1283–1287. [PubMed] [Google Scholar]
  5. Farrar W. L., Humes J. L. The role of arachidonic acid metabolism in the activities of interleukin 1 and 2. J Immunol. 1985 Aug;135(2):1153–1159. [PubMed] [Google Scholar]
  6. Fleisher L. N. Effects of inhibitors of arachidonic acid metabolism on endotoxin-induced ocular inflammation. Curr Eye Res. 1988 Mar;7(3):321–327. doi: 10.3109/02713688809047038. [DOI] [PubMed] [Google Scholar]
  7. Fleisher L. N., McGahan M. C. Endotoxin-induced ocular inflammation increases prostaglandin E2 synthesis by rabbit lens. Exp Eye Res. 1985 May;40(5):711–719. doi: 10.1016/0014-4835(85)90140-x. [DOI] [PubMed] [Google Scholar]
  8. Guivernau M., Terragno A., Dunn M. W., Terragno N. A. Estrogens induce lipoxygenase derivative formation in rabbit lens. Invest Ophthalmol Vis Sci. 1982 Aug;23(2):214–217. [PubMed] [Google Scholar]
  9. Imanishi M., Kawamura M., Akabane S., Matsushima Y., Kuramochi M., Ito K., Ohta M., Kimura K., Takamiya M., Omae T. Aspirin lowers blood pressure in patients with renovascular hypertension. Hypertension. 1989 Nov;14(5):461–468. doi: 10.1161/01.hyp.14.5.461. [DOI] [PubMed] [Google Scholar]
  10. Kass M. A., Holmberg N. J. Prostaglandin and thromboxane synthesis by microsomes of rabbit ocular tissues. Invest Ophthalmol Vis Sci. 1979 Feb;18(2):166–171. [PubMed] [Google Scholar]
  11. Keeting P. E., Lysz T. W., Centra M., Fu S. C. Prostaglandin biosynthesis in the rat lens. Invest Ophthalmol Vis Sci. 1985 Aug;26(8):1083–1086. [PubMed] [Google Scholar]
  12. Miyake K., Maekubo K., Miyake Y., Nishi O. Pupillary fibrin membrane. A frequent early complication after posterior chamber lens implantation in Japan. Ophthalmology. 1989 Aug;96(8):1228–1233. doi: 10.1016/s0161-6420(89)32746-1. [DOI] [PubMed] [Google Scholar]
  13. Nagineni C. N., Bhat S. P. Human fetal lens epithelial cells in culture: an in vitro model for the study of crystallin expression and lens differentiation. Curr Eye Res. 1989 Mar;8(3):285–291. doi: 10.3109/02713688908997570. [DOI] [PubMed] [Google Scholar]
  14. Nishi O. Fibrinous membrane formation on the posterior chamber lens during the early postoperative period. J Cataract Refract Surg. 1988 Jan;14(1):73–77. doi: 10.1016/s0886-3350(88)80068-3. [DOI] [PubMed] [Google Scholar]
  15. O'Neill L. A., Barrett M. L., Lewis G. P. Induction of cyclo-oxygenase by interleukin-1 in rheumatoid synovial cells. FEBS Lett. 1987 Feb 9;212(1):35–39. doi: 10.1016/0014-5793(87)81551-x. [DOI] [PubMed] [Google Scholar]
  16. Powell W. S. Rapid extraction of oxygenated metabolites of arachidonic acid from biological samples using octadecylsilyl silica. Prostaglandins. 1980 Nov;20(5):947–957. doi: 10.1016/0090-6980(80)90144-6. [DOI] [PubMed] [Google Scholar]
  17. Ringens P., Mungyer G., Jap P., Ramaekers F., Hoenders H., Bloemendal H. Human lens epithelium in tissue culture: biochemical and morphological aspects. Exp Eye Res. 1982 Oct;35(4):313–324. doi: 10.1016/0014-4835(82)90094-x. [DOI] [PubMed] [Google Scholar]
  18. Santos B. A., Pastora R., DelMonte M. A., O'Donnell F. E., Jr Lens epithelial inhibition by PMMA optic: implications for lens design. J Cataract Refract Surg. 1986 Jan;12(1):23–26. doi: 10.1016/s0886-3350(86)80050-5. [DOI] [PubMed] [Google Scholar]
  19. Sawa M., Tsurimaki Y., Tsuru T., Shimizu H. New quantitative method to determine protein concentration and cell number in aqueous in vivo. Jpn J Ophthalmol. 1988;32(2):132–142. [PubMed] [Google Scholar]
  20. Taylor L., Menconi M., Leibowitz M. H., Polgar P. The effect of ascorbate, hydroperoxides, and bradykinin on prostaglandin production by corneal and lens cells. Invest Ophthalmol Vis Sci. 1982 Sep;23(3):378–382. [PubMed] [Google Scholar]
  21. Wålinder P. E., Olivius E. O., Nordell S. I., Thorburn W. E. Fibrinoid reaction after extracapsular cataract extraction and relationship to exfoliation syndrome. J Cataract Refract Surg. 1989 Sep;15(5):526–530. doi: 10.1016/s0886-3350(89)80109-9. [DOI] [PubMed] [Google Scholar]
  22. van Dorp D. A., Jouvenaz G. H., Struijk C. B. The biosynthesis of prostaglandin in pig eye iris. Biochim Biophys Acta. 1967 Apr 4;137(2):396–399. doi: 10.1016/0005-2760(67)90117-8. [DOI] [PubMed] [Google Scholar]

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