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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Arthritis Rheumatol. 2016 Oct;68(10):2540–2549. doi: 10.1002/art.39743

Figure 3. Progenitors expressing CD31, a marker of early blood vessels, have high levels of IFI16 and CENP-A expression.

Figure 3

EB day 12 cultures were embedded, cryosectioned and double stained with CD31 and antibodies against either IFI16 or CENP-A as described in the Methods section. Top panel (10×): IFI16 (green), CD31 (red), and a merged image with DAPI (blue nuclear stain) shows enrichment of IFI16 in cells expressing CD31 in day 12 EB's. Middle panel (63×): Higher magnification of the image shown in the top panel. Bottom panel (63×): CENP (red) is enriched in CD31 expressing cells (green). A merged image with DAPI (blue nuclear stain) is also shown. Scale bar represents 100 microns (top panel) and 20 microns (middle and lowest panels).