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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Pharm Res. 2016 Jul 18;33(11):2713–2721. doi: 10.1007/s11095-016-1998-x

Figure 6. PGP expression is upregulated in primary monocyte derived macrophages that have been polarized to the M2 phenotype.

Figure 6

Peripheral blood mononuclear cells were obtained from healthy subjects, and enriched for monocytes via negative selection with RosetteSep. Monocytes were differentiated for 6 days with M-CSF, then treated with cytokines to polarize to the M1 or M2 phenotypes. (A) Cell lysates were collected, and probed for PGP via western blotting with a rabbit polyclonal to PGP antibody, with an anti-Rb secondary antibody. (B) Densitometry: Blot density was analyzed using Image Studio, with sample density normalized to the loading control. Sample density was normalized to the unstimulated condition. Data represents averaged densitometry readings of 3 blots. Comparisons between groups were made via repeated measures ANOVA with Tukey's multiple comparison test. Data represents averaged densitometry readings for 3 blots.