Fig. 1.

Effects of maternal adiponectin on fetal body weight. (a) Schematic diagram showing the production of Adipoq^−/+ fetuses. For this, 10–12 week-old nulliparous Adipoq^−/− and WT female mice were mated with WT or Adipoq^−/− male mice. (b) Maternal body weight. (c) Gestational weight gain was calculated by the difference in body weight between E0.5 and E18.5 (with pups). (d) Maternal body fat, (e) litter size and (f) fetal body weight were determined after removal of fetuses through Caesarean section at E18.5. (g) At E15, Adipoq^−/− dams (n=6) were transduced with purified Ad-gfp or Ad-Adipoq through tail vein injection. Fetal samples were collected and weighed at E18.5. (h,k) Blood glucose, (i) NEFA and (j) TG levels were measured using glucose oxidase or assay kits. (l) Fetal:placental weight ratios were calculated using samples at E18.5. (b, d) Maternal body composition was determined by EchoMRI. (b–e, h–j) n=8–10; (f, g, l) n=45–72; (k) n=12. *p<0.05, **p<0.01, ***p<0.001