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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Diabetologia. 2016 Aug 5;59(11):2417–2425. doi: 10.1007/s00125-016-4061-x

Fig. 4.

Fig. 4

Adiponectin enhanced the IGFBP-1 expression in trophoblast cells via PPARα. (a) IGFBP-1 levels in primary trophoblast cells treated overnight with a PPARα agonist WY14643 (5 µmol/l) or antagonist MK886 (10 µmol/l) were analysed by western blotting. (b) Adiponectin was ectopically expressed in Ad-Adipoq-transduced Fao cells. Insert wells containing transduced Fao cells were co-cultured overnight with trophoblast cells. MK886 was then added to one group of adiponectin-treated cells (Adipoq). IGFBP-1 protein levels in trophoblast cells were analysed by western blotting (n=6). RU, relative units. *p<0.05 vs control cells (Con)