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. 2016 May 27;2(5):685–700. doi: 10.1016/j.jcmgh.2016.05.010

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Alcohol-induced acrolein and ER stress leads to steatosis, hepatocyte apoptosis, and liver injury in mice livers. (A) Hepatic steatosis (by H&E; magnification, 40×) and Oil Red O staining; magnification, 20×). (B) Apoptosis by TUNEL staining (magnification, 20×), with quantification of apoptosis. Means ± SEM, n = 6 mice. ***P < .001 compared with control by Student t test. (C) Liver injury: serum ALT and AST. Means ± SEM, n = 6 mice. **P < .01 and ***P < .001 compared with control by the Student t test. C, control; E, alcohol.