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Binding of pentalenolactone F (2) and 6,7-dihydropentalenolactone F (7) to wild-type PntM and mutants.
| Protein | KD (μM) | Protein | KD (μM) |
|---|---|---|---|
|
| |||
| PntM - wt | 7.2±0.8 (2) | PntM - wt | 170±17 (7) |
| PntM-F232A | 270±85 (2) | PntM-M81A | 340±90 (2) |
| PntM-F232G | 225±45 (2) | PntM-M81C | 43±3 (2) |
| PntM-F232L | 160±12 (2) | PntM-M77S | 115±8 (2) |
| PntM-M81C-BME | 31±1 (2) | ||
