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. Author manuscript; available in PMC: 2017 Sep 28.
Published in final edited form as: J Am Chem Soc. 2016 Sep 14;138(38):12527–12533. doi: 10.1021/jacs.6b06823

Figure 7.

Figure 7

Site-directed mutagenesis of BM3-HStar to improve yield and selectivity of cyclopropanation of acrylamide 1 with EDA. Reactions were performed with 10 µM enzyme, 10 mM of 1, 20 mM of EDA, and 10 mM of Na2S2O4 in 100 mM KPi buffer (pH = 8.0). Yields and diastereoselectivity were determined by GC calibrated for 2. Enantioselectivity was determined by chiral SFC (See Supporting Information for GC yield determination).