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. Author manuscript; available in PMC: 2017 Sep 28.
Published in final edited form as: J Am Chem Soc. 2016 Sep 14;138(38):12527–12533. doi: 10.1021/jacs.6b06823

Figure 8.

Figure 8

Total turnover numbers (TTNs) for cyclopropanation of 1 with whole cells expressing BM3-HStar variants. Reactions were performed with whole cells expressing the enzyme (resuspended in M9-N media to OD600 = 10), 10 mM of 1, and 20 mM of EDA. TTN = [product]/[enzyme]; enzyme concentration in whole-cell suspension was determined by ferrous expression assay (See Supporting Information for assay procedure); product concentration at the end of the reaction was determined by GC calibrated for 2.