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The association of GST-β-adducin tail with band 3 is not affected by the absence of the first 65 amino acids of cdb3. His-tagged kidney-cdb3 (triangles) or BSA (open squares) was immobilized on nickel beads and incubated with increasing concentrations of GST-β-adducin tail. Beads were pelleted, washed 5 times, and eluted with 250 mM imidazole in PBS. GST activity was then quantified as a measure of the amount of bound adducin. (a.u. represents arbitrary units).
