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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Biochem J. 2016 Jul 19;473(19):3147–3158. doi: 10.1042/BCJ20160328

Figure 5.

Figure 5

Mutation of cdb3 does not affect other protein functions. (A) Dot blot analysis of GST-D3D4-ankyrin interaction with wild-type and mutant cdb3. 1) Empty bead control, 2) wild-type cdb3, or 3) mutant cdb3 were immobilized on nickel beads and incubated with increasing concentrations of GST-D3D4-ankyrin. After washing and elution of proteins with 250 mM imidazole, eluted proteins were dot blotted on to nitrocellulose and probed with anti-GST antibody. (B) Effect of wild type and mutant cdb3 on GAPDH activity in solution. Wild-type erythrocyte band 3 has already been established to bind and inhibit GAPDH [39], whereas BSA has no effect on GAPDH activity. Data points represent mean ± S.D., n = 4.