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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Biochem J. 2016 Jul 19;473(19):3147–3158. doi: 10.1042/BCJ20160328

Figure 6.

Figure 6

Cdb3 mutants that cannot bind adducin retain normal structural properties. (A) Comparison of the pH-dependent conformational changes of wild-type and mutant cdb3. The intrinsic fluorescence (emission at 335 nm) of purified recombinant wild-type and mutant cdb3 was measured following excitation 290 nm and plotted as a function of pH. Data points represent mean ± S.D., n = 2. (B) Comparison of the circular dichroism spectra of wild-type and mutant cdb3. The CD spectrum of wild-type and mutant cdb3 was monitored in 50 mM sodium phosphate, 50 mM sodium borate, 70 mM NaCl, pH 7.4 at 24°C using a Jasco Model J810 CD spectropolarimeter.