Table 1.
Band 3 sequence labeled by photo-activated transfer of biotin from sulfo-SBED-adducin to cdb3.1
| Digestion enzyme |
Sulfo-SBED α-adducin tail |
Sulfo-SBED β-adducin tail |
|---|---|---|
| Trypsin | 247LQEAAELEAVELPVPIR263 | 247LQEAAELEAVELPVPIR263 |
| Chymotrypsin | 251AELEAVELPVPIRF264 | 254EAVELPVPIRF264 |
| Proteinase K | 245VRLQEAAELEAVELPVPIRF264 | 245VRLQEAAELEAVELPVPIRF264 |
| Endo Lys-C + Trypsin |
248QEAAELEAVEL258 | 248QEAAELEAVEL258 |
1
Sulfo-SBED α- or β-adducin tail domain was allowed to bind cdb3, after which the biotin label in sulfo-SBED was transferred to cdb3 by illumination with UV light. Following proteolysis as indicated below, biotinylated peptides were isolated on an avidin column and analyzed by MALDI-TOF mass spectrometry.