Supplementary Figure 1.

Lgr5–eGFP–ires–creER–mediated lineage tracing across time. Timed pregnant females were given tamoxifen when embryos were E10.5 (top row), E12.5 (middle row), or E14.5 (bottom row), and all embryos were collected at E16.5. E10.5 and E12.5 lineage tracing was evident in the stomach and intestine, whereas less lineage tracing was seen in the stomach and more was seen in the intestine when tamoxifen was given at E14.5. Lineage tracing was performed with Rosa26–LacZ and X-gal staining is shown in blue. Scale bar: 200 μm.