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Agarose gel electrophoresis showing monoplex and multiplex PCR-amplified products. M, 100-bp DNA marker; lane 1, Salmonella enteritidis ATCC13076; lane 2, L. monocytogenes ATCC19111; lane 3, E. coli O157:H7 (HCMUS); lane 4, 16S rRNA of bacterial DNA; lane 5, a mixture of the three DNA templates of pathogens before the optimizing; lane 6, the multiplex PCR-optimized conditions with four targets; and NC, negative control
