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. 2016 Sep 24;6(2):205. doi: 10.1007/s13205-016-0523-6

Table 2.

Primers selected for the multiplex PCR in this study

Target pathogen Primer sequence (5′ → 3′) Gene PCR product (bp) Reference
Escherichia coli
O157:H7
gagcgaaataatttatatgtg Stx1, stx2 518 Toma et al. (2003)
tgatgatggcaattcagtat
Salmonella spp. acagtgctcgtttacgacctgaat invA 284 Rahn et al. (1992)
agacgactggtactgatcgataat
L. monocytogenes cgcaacaaactgaagcaaagg hlyA 404 Shaw et al. (2004)
ttggcggcacatttgtcac
Bacterial DNA gtattgaaagctctggcgg 16S rRNA 654 Qu et al. (2006)
tcgcttagtctctgaaccc