Table 4.
Multiplex PCR results of three pathogens from artificially inoculated three food sample matrices
| Pathogens | Incubation time (h) | CFU/mL | Multiplex PCR results detection in food samples | ||
|---|---|---|---|---|---|
| Raw pork | Chicken | Vegetable salads | |||
| E. coli O157:H7 (HCMUS) (stx) | 12 | 0 | − | − | − |
| 100 | − | − | − | ||
| 101 | + | + | + | ||
| 102 | + | + | + | ||
| 18 | 0 | − | − | − | |
| 100 | − | − | − | ||
| 101 | + | + | + | ||
| 102 | + | + | + | ||
| 24 | 0 | − | − | − | |
| 100 | − | − | − | ||
| 101 | + | + | + | ||
| 102 | + | + | + | ||
| S. enterica ATCC13076 (invA) | 12 | 0 | − | − | − |
| 100 | − | − | − | ||
| 101 | + | + | + | ||
| 102 | + | + | + | ||
| 18 | 0 | − | − | − | |
| 100 | − | − | − | ||
| 101 | + | + | + | ||
| 102 | + | + | + | ||
| 24 | 0 | − | − | − | |
| 100 | − | − | − | ||
| 101 | + | + | + | ||
| 102 | + | + | + | ||
| L. monocytogenes ATCC19111 (hlyA) | 12 | 0 | − | − | − |
| 100 | − | − | − | ||
| 101 | + | + | + | ||
| 102 | + | + | + | ||
| 18 | 0 | − | − | − | |
| 100 | − | − | − | ||
| 101 | + | + | + | ||
| 102 | + | + | + | ||
| 24 | 0 | − | − | − | |
| 100 | − | − | − | ||
| 101 | + | + | + | ||
| 102 | + | + | + | ||