Fig. 9.

BNP (i.t.) suppressed BmK I-induced inflammatory pain-related behaviors. a: Rat flinch behavior was attenuated by pretreatment of 10 μl saline or BNP(1, 2 or 3 μg in saline) at 2 h before BmK I administration. b: Rat flinch behavior was attenuated by pretreatment of 10 μl saline or BNP(2 μg in saline) at 0.5, 2, 3, 4 h before BmK I administration. c: Suppression of total number of the rat paw flinches by 10 μl saline or BNP(1, 2 or 3 μg in saline) during 2 h after BmK I injection. d: Suppression of total number of the rat paw flinches by 10 μl saline or BNP(2 μg in saline) during 2 h after BmK I injection. e: Suppression of total number of paroxysmal pain-like behaviors by 10 μl saline or BNP(2 μg in saline) during 2 h after i.pl. BmK I injection. f: Suppression of duration of lifting and licking behaviorsby 10 μl saline or BNP(2 μg in saline) during 2 h after i.pl. BmK I injection. Ipsilateral mechanical hyperalgesia (g), contralateral mechanical hyperalgesia (h) and ipsilateral thermal hyperalgesia (i) were suppressed by 10 μl saline or BNP(2 μg in saline) pretreatment for 0.5, 2, 3, 4 h. j: There was no difference of conltralateral basal thermal latency among five groups. Rat hindpaw injected with BmK I was considered as ipsilateral side, and the other side was named as contralateral side. All data were showed as mean ± S.E.M. (a, c: n = 3; b, d-j: n = 7). *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05, ##P < 0.01, ###P < 0.001, compared with BmK I + saline group