Fig. 3.

Collapse of the IF network due to high GFP–GFAPδ expression. a Stills from a representative live cell imaging experiment. U343MG cells were transfected with GFP–GFAPδ and imaged for 48 h. The GFP–GFAPδ was initially incorporated into the IF network (arrows at t = 12 h), but as the amount of GFP–GFAPδ increased over time, it eventually caused a collapse of the network (t = 18 h). During the process of collapsing, thicker and shorter filamentous structures are visible in the cell, which are moving into the direction of the collapsed network (arrowheads in t = 30 h). b These small filaments sometimes co-localized with vimentin in U251MG cells as well (yellow arrow). Scale bars represent 20 μm. See also supplemental Movie 1 and 2