Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 30;7:1563. doi: 10.3389/fmicb.2016.01563

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Stone, Kroukamp, Korber, McKelvie and Wolfaardt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Closed-loop desiccation metabolic measurements. Mixed-culture inocula were incubated well into stationary phase, with bentonite, sand, polyethylene glycol (PEG) and tap water, respectively. Inocula were transferred to microcosm tubes [15 mL per tube, in triplicate for low relative humidity (RH) and high RH], and desiccated overnight. Microcosm tubes were transferred to incubators maintained at low (30%) and high (75%) RH. After 3 days of incubation at the respective RH, tubes were connected to a carbon dioxide evolution monitoring system (CEMS) and CO₂ accumulation was measured in the closed-loop system.