Table 2.

Summary of second‐order rate constants for MTSEA modification

	MTSEA k2 (m −1 s−1)	MTSEA + GABA k2 (m −1 s−1)	MTSEA + propofol k2 (m −1 s−1)
α1(D43C)β2γ2	70,030 ± 9,629 (6)	34,530 ± 10,420 (6)*	38,860 ± 8,437 (7)
α1(T47C)β2γ2	6,216 ± 784.8 (8)	3,892 ± 873.6 (5)	6,049 ± 1,271 (6)
α1(F64C)β2γ2	1,944,000 ± 460,500 (4)†	24,660 ± 9,129 (4)*	1,832,000 ± 205,400 (5)

Data are provided as the mean ± SEM of (n) number of experiments. The second‐order rate constants are calculated by dividing the rate of decay, obtained from the exponential fits to data that generated the rate of MTSEA modification data, by the concentration of MTSEA used. The concentration of MTSEA used was 100 μm for α1(D43C)β2γ2 and α1(T47C)β2γ2 receptors, and 10 μm for α1(F64C)β2γ2 receptors. ^*Significant difference (P < 0.05) from one‐way ANOVA with post hoc Tukey's comparison between the presence or absence of saturating concentrations of GABA or activating concentrations of propofol. ^†Significant difference (P < 0.05) from one‐way ANOVA with post hoc Tukey's comparison between MTSEA k2 of different mutants.