Figure 6. Ca_V2.2 cell surface expression: effect of gabapentin .

Cell surface expression of bungarotoxin binding site (BBS) tagged Ca_V2.2 labelled with α‐bungarotoxin (BTX) coupled to AF488 dye (green). Left panel: examples of N2a cells transfected with Ca_V2.2/β1b/α₂δ‐1 in the absence (left), and presence (right) of gabapentin (100 μm for 24 h). Right panel: mean (± SEM) data for cell surface expression of Ca_V2.2, for cells expressing Ca_V2.2/β1b/α₂δ‐1 (black‐filled and open bars), Ca_V2.2/β1b/α₂δ‐1 R241A (a mutant α₂δ‐1 that does not bind gabapentin; red‐filled and open bars) in the absence (filled bars), and presence (open bars) of gabapentin 100 μm for 24 h. Data are taken from a recent study (Cassidy et al. 2014). The number of cells measured is indicated on the bars, ^*** P<0.001, Student's t test.