Table 1. qRT-PCR detection of transgene overexpression.
| U251 pcDNA | U251 pTRPZ | U251-NS pTRPZ | ||
|---|---|---|---|---|
| (−) Dox | (+) Dox | (+) Dox | ||
| Vector | N/E | 0 | 0 | 0.1 |
| E1 | 1.6 | 0.1 | 12.7 | 133.3 |
| E2 | 23.7 | 0.1 | 3.4 | 74.2 |
| E3 | 81 | N/E | N/E | 46.7 |
| E4 | 1.6 | N/E | N/E | N/E |
| E5 | 22.4 | 0.1 | 4.5 | 1.9 |
| E6 | 29.6 | 0.1 | 8.1 | N/E |
| E7 | 1.8 | 0 | 3.3 | 43.8 |
| E8 | 1.6 | 0.1 | 5.2 | 14.2 |
| E9 | 1.4 | N/E | N/E | N/E |
| E10 | 49.5 | N/E | N/E | 55.2 |
| E11 | 153.9 | 0.1 | 54.9 | 198 |
| E13 | 24.2 | 0.1 | 25.4 | 6.6 |
| E14 | 10.6 | N/E | N/E | 46.7 |
| E15 | 62.5 | 0.1 | 9.3 | 85.4 |
| E18 | N/E | 0.2 | 6.9 | 142.3 |
Values are ratio of FLAG-EFEMP1/ACTB times 1000
N/E: Not examined