Figure 4. Persisters’ and starved cells’ proteome are shaped by the same cue.

The experimental and data analysis procedure is described in the gray boxes step by step.

1. PCA plot of the Escherichia coli proteomes in different conditions and time points. Each point represents a proteome in a different state. The distances between points are inversely correlated with the similarity between proteomes (i.e. proteomes with higher correlation coefficient have a shorter distance between each other), calculated based on differences in the expression level of each quantified protein. Green disk: cells growing on glucose, green square: cells growing on fumarate, gray disks: cells entering persistence after glucose‐to‐fumarate switch, open circles: cells entering starvation from glucose, open squares: cells entering starvation from fumarate. Time series are indicated by gray color gradients.
2. The progression of changes upon entry into starvation and entry into persistence happens in the same direction in the two‐dimensional space, indicating that the same cue shapes these proteomes.