Table 1.

Primers used to perform RT-PCR of miRNAs

miRNA name	RT primers (5’→3’)	PCR primers (5’→3’)
hsa-miR-193b-3p	5’-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAGCGGGA-3’	GSP: 5’-GGGAACTGGCCCTCAAAG-3’
		R: 5’-GTGCGTGTCGTGGAGTCG-3’
hsa-miR-16-2-3p	5’-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTAAAGC-3’	GSP: 5’-GGGGACCCAATATTACTGTGCT-3’
		R: 5’-GTGCGTGTCGTGGAGTCG-3’
hsa-miR-4534	5’-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAGACCC-3’	GSP: 5’-GGGAAGGGATGGAGGAGG-3’
		R: 5’-GTGCGTGTCGTGGAGTCG-3’
hsa-miR-505-3p	5’-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAGGAAA-3’	GSP: 5’-GGCGTCAACACTTGCTGG-3’
		R: 5’-GTGCGTGTCGTGGAGTCG-3’
hsa-miR-4441	5’-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTACAAT-3’	GSP: 5’-GGGGGATACAGGGAGGAGA-3’
		R: 5’-GTGCGTGTCGTGGAGTCG-3’
hsa-miR-5187-3p	5’-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCTGAGG-3’	GSP: 5’-GGGGGGACTGAATCCTCTTT-3’
		R: 5’-GTGCGTGTCGTGGAGTCG-3’
hsa-miR-200a-3p	5’-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACATCG-3’	GSP: 5’-GGGGGTAACACTGTCTGGTAA-3’
		R: 5’-GTGCGTGTCGTGGAGTCG-3’
hsa-miR-194-5p	5’-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCCACAT-3’	GSP: 5’-GGGGGTGTAACAGCAACTCC-3’
		R: 5’-GTGCGTGTCGTGGAGTCG-3’
hsa-miR-130b-3p	5’-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACATGCCC-3’	GSP: 5’-GGGGGTGCAATGATGAAA-3’
		R: 5’-GTGCGTGTCGTGGAGTCG-3’
hsa-miR-125a-3p	5’-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGGCTCC-3’	GSP: 5’-GGGGACAGGTGAGGTTCTTG-3’
		R: 5’-GTGCGTGTCGTGGAGTCG-3’
hsa-miR-3168	5’-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGTCTGA-3’	GSP: 5’-GGGGGGGGAGTTCTACAGT-3’
		R: 5’-GTGCGTGTCGTGGAGTCG-3’
hsa-miR-361-5p	5’-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGTACCC-3’	GSP: 5’-GGGGGTTATCAGAATCTCCAG-3’
		R: 5’-GTGCGTGTCGTGGAGTCG-3’
U6	5’-CGCTTCACGAATTTGCGTGTCAT-3’	F: 5’-GCTTCGGCAGCACATATACTAAAAT-3’
		R: 5’-CGCTTCACGAATTTGCGTGTCAT-3’

Note: GSP is the specific primers. F: forward primer; R: reverse primer.