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. 2016 Sep 1;6(9):1906–1921.

Figure 4.

Figure 4

Differentiated subpopulation of DsRed-LLCcm contributes to tube formation. A: Immunofluorescence analysis of CD31 in puromycin-selected miPS-LLCcm cells. Original magnification ×60. B: Incomplete tube formation of puromycin selected miPS-LLCcm cells. Puromycin selected cells were seeded on Matrigel and stained with CD31 antibody (red). Original magnification ×20. C: Tube formation capacity of spheres. Sphere forming self-renewing miPS-LLCcm cells, or cells after 2 days differentiation of spheres were subjected to in vitro tube formation assays. Scale bar: Day 0: 200 µm; Day 2: 50 µm; Tube formation Original magnification ×2.