Figure 5.

Down-expression of HRAS inhibits gastric cancer progression in vivo. A. Effect of HRAS on the growth of MKN28 cells inoculated into nude mice. BALB/c-nu mice were subcutaneously injected with MKN28/RNAi-vector or MKN28/HRAS-RNAi cells. Tumor volume and weight were monitored over time as indicated, and the tumor was excised after 25 days. HRAS down-expression causes a decrease in tumor volume. B. Tumor growth curve upon implantation. For indicated comparisons, **P < 0.01. C. Body weight changes in mice subcutaneously injected with MKN28/RNAi-vector or MKN28/HRAS-RNAi cells. There was no significant difference in body weight between RNAi-vector and HRAS-RNAi group. D. Tumor sections were analyzed by immunohistochemistry for detection of Ki67 expression in each group of nude mice. Apoptotic cells were examined by TUNEL staining. Each image was representative of six independent mice.