Figure 6.

HRAS siRNA inhibits gastric cancer induced angiogenesis in vivo. A. The expression levels of tumor induced angiogenesis markers including CD31 and VEGFA from the tumor tissues of MKN28/HRAS-siRNA group were lower than that of control group by immunohistochemistry assay. B. The proteins were extracted from tumor xenografts and were subjected to western blot for measuring phosphor-PI3K, total-PI3K, phosphor-AKT, total-AKT, phosphor-mTOR, total-mTOR, phosphor-GSK-3β, total-GSK-3β, phosphor-NF-κB p65, total-NF-κB p65, phosphor-Raf-1, total-Raf-1, phosphor-ERK1/2, total-ERK1/2, phosphor-MEK1/2, total-MEK1/2, phosphor-p38 MAPK and total-p38 MAPK, respectively.