Figure 3.
miR-302b inhibits Mcl-1 gene expression via directly binding at the Mcl-1 3’UTR. Alignment of miR-302b with the insert derived from the Mcl-1 3’UTR (A). Cells (1 × 105) were transfected with a Mcl-1 luciferase reporter vector and miR-302b GFP-expression vector. Both firefly luciferase and Renilla luciferase activities were measured as described in the procedure. Firefly luciferase activity was then normalized with Renilla luciferase activities in the same well in MMC1 (B) and MMC2 (C) respectively. The experiments were performed 3 times separately in triplicates and data is represented as ± SEM, and values *P < 0.05 as compared to control-miR vector.