Figure 4.

miR-302b inhibitor restored ephrin-A1-mediated proliferation inhibition in MMCs. MMC proliferation as determined by WST-1 assay, wherein control = untreated MMCs and Sc-control = MMC transfected with miR-302b scrambled miRNA (A). miR-302b inhibitor restored ephrin-A1-mediated attenuation of tumorsphere growth in MMC. MMCs were transfected with or without miR-302b and miR-302b inhibitor or Mcl-1 siRNA plus miR-302b as indicated and activated with or without ephrin-A1 and tumorsphere formation was determined (B, C). Data presented is the representatives of three independent observations performed at different times. All experiments were performed 3 times separately and data is represented as ± SEM, and values *p < 0.05 as compared to control; and $p < 0.05 as compared to Sc-sequence; **p < 0.05 as compared to con-siRNA; and NS = not significant when compared to only miR-302b and ephrinA1 alone.