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Combination trial of miR-inhibitor and SIRT5 siRNA Primary hepatocytes co-transfected with SIRT5 siRNA and miR-19b inhibitor or inhibitor negative control, (A) urea concentration in hepatocytes supernatant was assayed by urea nitrogen assay kit. Data are presented as mean ± SD (*P < 0.05, **P < 0.01,n = 6); (B) The protein expression of SIRT5 and CPS1 were simultaneously detected by western blotting. Data are presented as mean ± SD (*P < 0.05, n = 6).
