Figure 6. Specific ADAMTS9 TSRs are O-fucosylated.

(a) Domain structure of ADAMTS9 and of the ADAMTS9 constructs used for mass spectrometry (hADAMTS9-N-L2 and hADAMTS9 TSR9-15). TSRs that carry a consensus sequence for O-fucosylation are shown in red. (b) Extracted ion chromatograms showing the relative amounts of the unmodified form (black line), O-fucose monosaccharide form (red line, fucose indicated by red triangle), and Glucoseβ1-3Fucose disaccharide form (blue line, glucose indicated by blue circle) of peptides from TSRs 5–9, TSRs11-13 and TSR15 of ADAMTS9 generated using the corresponding masses listed in Appendix Table S1. Note that lack of data for TSRs 1, 4, and 14 does not indicate that they are unmodified, but that the ions corresponding to those sites were not detected. (*=contaminating peptide).