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. 2016 Sep 30;6:34501. doi: 10.1038/srep34501

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) The strategy for the conditional expression of Cripto-1 and Luc transgenes. Please see the Materials and Methods for additional details on the strategy for the conditional expression of the transgenes. (B–E) Cre-mediated excision efficiency in embryonic hearts. (B,D) Whole-mount blue-stained embryonic hearts. (C,E) Histological sections of E11.5 (C) and E13.5 (E) embryonic hearts. The ventricular surface is indicated with a “*”, the atrial appendage is indicated with a “#”, and arteries are indicated with a “*”. (F) RT-PCR analysis using human-specific primer for human Cripto-1 transgene expression in RCLG/EIIa-Cre transgenic embryos and embryonic hearts. The cropped gels are used in Fig. 1F, and the full-length gel images are available in Supplementary Figure S28. The gels have been run under the same experimental conditions. (G) Western blot for the Cripto-1 protein in RCLG/EIIa-Cre transgenic embryos and their hearts. For (F,G), lane 1: E7.5+RCLG/Cre; lane 2: E7.5+Cre; lane 3: E8.5+RCLG/Cre; lane 4: E8.5+Cre; lane 5: E9.5+RCLG/Cre; lane 6: E9.5+Cre; lane 7: E10.5+RCLG/Cre; lane 8: E10.5+Cre; lane 9: E11.5+RCLG/Cre; lane 10: E11.5+Cre; lane 11: E12.5+RCLG/Cre; lane 12: E12.5+Cre; lane 13: E13.5+RCLG/Cre; lane 14: E13.5+Cre; lane 15: E14.5+RCLG/Cre; lane 16: E14.5+Cre; lane 17: E15.5+RCLG/Cre; lane 18: E15.5+Cre. The cropped blots are used in Fig. 1G, and the quantification of Cripto-1 expression and the full-length gel images are available in Supplementary Figures S6 and S28, respectively. The blots have been run under the same experimental conditions.