Table 1. Genotype frequency of progeny from the mating of RCLG mice and EIIa-Cre mice.
| Stage | No. of litters | Genotype (No. of offspring with the indicated genotype) | Total | ||||
|---|---|---|---|---|---|---|---|
| G1 | G2 (hemorrhage) | G2 (resorbed) | G2 (pale body surface) | G2 (no abnormal appearance) | |||
| E12.5 | 17 | 64 | 0 | 0 | 0 | 89 | 153 |
| E13.5 | 15 | 53 | 0 | 0 | 0 | 75 | 128 |
| E14.5 | 29 | 103 | 35 | 48 | 37 | 39 | 262 |
| E15.5 | 23 | 70 | 13 | 75 | 18 | 27 | 203 |
| E16.5 | 16 | 52 | 4 | 49 | 6 | 14 | 125 |
| E17.5 | 9 | 25 | 1 | 35 | 1 | 3 | 65 |
| Postnatal | 20 | 85 | 0 | 0 | 0 | 0 | 85 |
RCLG transgenic mice used here were heteroygous or homozygous, while EIIa-Cre mice used here were homozygous. The genotypes of offspring were determined by the combinational use of ex vivo bioluminescence and fluorescence imaging, as verified by PCR-based genotyping. The genetic background was FVB inbred mice. Genotype-1 (G1): Cre (control embryo), genotype-2 (G2): RCLG/Cre (mutant embryo).