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. 2016 Sep 30;6:34505. doi: 10.1038/srep34505

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Whole cell lysate was prepared from cells treated with 1 μM DAPT for 12 h. Equal amounts of cellular proteins were subjected to immunoblot analysis with anti-APP antibody Y188 (a) or 82E1(c). Longer (30 sec) and shorter (5 sec) exposure images are shown in panel a. β-actin served as an internal standard. Levels of α-CTF and/or β-CTF shown in panels a and c were quantified and presented in panels b and d, respectively. Data represent means ± s.d. (n = 6). Statistical analysis was performed by one-way ANOVA with Tukey-Kramer post hoc test (**P < 0.01).