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. 2016 Sep 30;6:34505. doi: 10.1038/srep34505

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Copyright © 2016, The Author(s)

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(a) Cells set up as in Fig. 2 were treated with 150 μM cycloheximide (CHX) for 2, 5, 8 or 12 h. Whole cell lysate was subjected to immunoblot to examine BACE1 and PSEN1 protein levels. (b) Relative changes in BACE1 protein levels in the cells treated with CHX were plotted based on panel (a). (c) Relative BACE1 protein levels in cells expressing the indicated gangliosides after the CHX treatment for 12 h. Data represent means ± s.d. (n = 8). *P < 0.05 by one-way ANOVA with Tukey-Kramer post hoc test.