Table 2. Pairwise agreement of ETBF status for four different PCR methods.
| Positive Percentage Agreement | Negative Percentage Agreement | Kappa | |||||
|---|---|---|---|---|---|---|---|
| (ref) | PPA | 95% CI | NPA | 95% CI | κ | P | |
| TaqMan qPCR | dPCR | 100.0% | (0.16,1.00) | 97.2% | (0.85,1.00) | 0.79 | 0.000 |
| SYBR qPCR | dPCR | 100.0% | (0.16,1.00) | 47.2% | (0.30,0.65) | −0.18 | 0.255 |
| Standard PCR | dPCR | 100.0% | (0.16,1.00) | 36.1% | (0.21,0.54) | −0.32 | <10−5 |
| SYBR qPCR | TaqMan qPCR | 100.0% | (0.29,1.00) | 48.6% | (0.31,0.66) | −0.10 | 0.553 |
| Standard PCR | TaqMan qPCR | 100.0% | (0.29,1.00) | 37.1% | (0.21,0.55) | −0.24 | <10−5 |
| Standard PCR | SYBR qPCR | 100.0% | (0.84,1.00) | 76.5% | (0.50,0.93) | 0.78 | 0.000 |
qPCR, quantitative PCR; dPCR, digital PCR; PPA, positive percentage agreement; NPA, negative percentage agreement; ref, reference method with most positive calls.