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. 2016 Sep 30;6:34558. doi: 10.1038/srep34558

Figure 1. The increase of hippocampal cellular senescence and γ-H2AX activity in Wip1 KO mice.

Figure 1

(A) Illustration of immunochemistry samples showing SABG-staining positive cells located in hippocampal CA1 and dentate gyrus (DG) subareas. (B) Comparison of the number of SABG-staining positive cells within hippocampal CA1 and DG subareas between Wip1 KO mice and their wildtype (WT) littermates (n = 5 mice for each group). SABG-staining positive cells located in hilus were merged with those in DG and presented together. The number of SABG-staining positive cells significantly increased in Wip1 KO mice when compared to that in their WT littermates. (C) Illustration of immunochemistry samples showing hippocampal γ-H2AX positive cells. (D) There were obviously more hippocampal γ-H2AX positive cells in Wip1 KO mice than in the control group (n = 6 mice for each group). (E) The sample of Western blotting assay for the detection of γ-H2AX and the total H2AX expression in hippocampal tissues. β-action was probed as the protein loading control of samples. (F) Wip1 KO mice exhibited the increase of γ-H2AX expression when compared to the control group. The γ-H2AX expression was normalized to the total H2AX. n = 6 samples for each group. **P < 0.01.