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. 2016 Sep 30;6:34558. doi: 10.1038/srep34558

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Illustration of hippocampal γ-H2AX positive cells in CUMS-exposed mice and their controls treated with fluoxetine or vehicle. (B) The increase of the number of hippocampal γ-H2AX positive cells was successfully prevented following fluoxetine treatment for 2 weeks (n = 5 mice for each group). (C) The expression of γ-H2AX and the total H2AX in hippocampal tissue was detected using Western blotting assay in CUMS-exposed mice and their controls treated with fluoxetine or vehicle. (D) The increased γ-H2AX expression was effectively prevented following fluoxetine treatment (n = 6 samples for each group). The expression of γ-H2AX was normalized to that of the total H2AX. **P < 0.01.