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. 2016 Sep 30;6:34345. doi: 10.1038/srep34345

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Copyright © 2016, The Author(s)

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(a) Ucp1 expression in offspring BAT of control (Con) and high-fat diet (HFD) dams at PND10 and PND21 (n = 4–6 per group). (b) mRNA expression of BAT-selective genes in BAT of Con and HFD offspring at weaning (n = 6 per group). (c,d) PPARα mRNA (c) and protein contents (d) in BAT of Con and HFD offspring at weaning (n = 6 per group). (e) Binding of PPARα to the Ucp1 promoter was elevated in HFD BAT as assayed by ChIP-PCR (n = 4 per group). Data are presented as Means ± S.E.M. *P < 0.05 as determined using a Student’s t-test.