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. 2016 Sep 30;6:34345. doi: 10.1038/srep34345

Figure 5. Maternal high-fat diet has long-term negative effects on BAT function of adult offspring at 4 months of age.

Figure 5

(a) mRNA expression of genes enriched in the BAT of control (Con) and high-fat diet (HFD) adult offspring (n = 7 each group). (b) Representative images of offspring BAT of Con and HFD feeding dams stained with H&E (top) and immunohistochemically with UCP1 antibody (bottom). (c) Oxygen consumption of isolated BAT from adult offspring under room temperature (n = 4 per group). (d) Dorsal interscapular surface temperature of Con and HFD offspring under room temperature and 4 °C for 6 h (n = 4–5 each group). Right (d), representative thermal images of mice under room temperature and 4 °C. (e) Oxygen consumption of Con and HFD adult offspring in basal status and injected with CL316,243 (n = 4 per group). Data are presented as Means ± S.E.M. *P < 0.05 as determined using a Student’s t-test or, where appropriate, two-way ANOVA and Tukey’ multiple comparison.